Background:

The client provided 6 Fab and scFv molecules. Activity and developability studies were needed to choose 3 molecules for further development.

Our solution: 

We cloned the codon-optimized gene sequences into our expression vectors. After transfection and selection, we got stable pools with titers >1g/L. The stable pools were used to generate material for the activity and developability studies. After the final 3 candidates were determined by the client, we did single cell cloning of the pools and got clone titers of 2.5-4.8g/L